Slicing

From NeuralNetoff

1.) Prepare 1x ACSF and Cutting Solution

2.) Set up heat bath and slicer

          - Put a beaker in a warm bath with ACSF in the beaker
          - Fill bath with water
          - Put the blade on the slicer, and fill platform with cutting solution
          - Turn vibratome and sink on

3.) Create a block ledge for slicing with agar gel

4.) Fill a container with ice

          - Fill a small beaker with cutting solution (~10 mL)
          - Put scissors, scalpel, bottom of beaker in the ice

5.) Turn oxygen on

          - Place oxygen tube in the beaker in the heat bath
          - Turn thermo on

6.) Obtain rat

          - Prepare slices by performing required cuts